what does this genotype mean? - (Oct/11/2009 )

URA3::MEL1UAS-MEL1TATA-lacZ

i know a new gene which encode the Lacz is inserted to the URA3. But i just wanna know whether the URA3 still working normally.

shashababy on Oct 11 2009, 03:55 PM said:

If its written with a ":" normally the gene is exchaned to the term behind the : anf therefore not functional anymore. If you want to be sure streak them onto a -URA plate.
Lg Jana