Freezing THP-1 - (Sep/29/2009 )
I always freeze 2x10(6)/ml. If you freeze down too high a concentration of cells they will not all be protected by the cryoprotectant and there will be a high percentage of dead cells when you thaw them anwyay
-SuMi-
SuMi on Jan 25 2010, 08:15 PM said:
I always freeze 2x10(6)/ml. If you freeze down too high a concentration of cells they will not all be protected by the cryoprotectant and there will be a high percentage of dead cells when you thaw them anwyay
Thanks for that, its just with other cell lines I always freeze down at a much higher (107) type concentration. Will try the 2 x 106 and see how it goes
-DSLJ-