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Problems with Quick change lightning multi site directed mutagenesis kit - (Sep/18/2009 )

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Dear warren,

This time i got 6 colonies in total for one reaction and 13 colonies for the second reaction........The colony number is still very low, in the kit they say you must expect 10-1000 colonies depending on the number and nature of primers and base composition of DNA.........if this colony number corresponds to the whole reaction then its not bad i think.....what do you say?

on each plate i plated 250ul of transformation reaction.......(each transformation reaction was plated on 3 plates)

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