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Please Help! Low intensity of Annexin V in Flow cytometry - (Sep/15/2009 )

:( Dear All,
I am in trouble. There is only 5 tests left and my annexin V FITC kit seems to be expired!
I obtain very low signal of Annexin V FITC and quite good signal of PI. Is there any trick so I can gain some results from the remaining samples?? Should I change the binding buffer or use it more concentrated?
in trouble please help:(:(:(


I think you answered your question. There is a reason for the designated expiration date. Don't take the chance and waste time - buy another vial.


-Jay Dela Cruz PhD-