fixing bacterial cells in ELISA - (Sep/14/2009 )
i am about to do ELISA for my Phd projects. In the assay i have to coat the plates with E.coli. i have read that some researchers fixed their bacterial cell with 1% formaldehyde prior to coating them onto the plate and dry them out overnight in 37C. can somebody explain to me why do the need to fix the cell first? wouldn't it will effect the result as compare to non fix-cell? and what are the effects of drying them out?
I'm not sure what the need for fixing other than to maybe prevent loss of membrane proteins. You would have to determine if fixing would increase the signal (given fixing does not alter epitope access) you're after.