I did intraperitoneal injection of tamoxifen to mice due to experiment needs. At begining the mice looked normal but gradually became feeble and even died 3 days later. When open the abdomen, there's a lot of white mass amnog internal organs. The chemical is dissolved in corn oil and stored at -20 C degree, and thawed before use. The solution is clear after thawing and no particles can be seen. The dosage delivered is 10mg/mouse, (body weight ~24gram on the day of injection), which should be less than LD50 of tamoxifen.
Has anybody encounterd a problem like this? What may go wrong? Help needed, thank you very much.
-lydialing-
Sounds like an inflammatory immune response, or that you punctured the gut!
-bob1-
my vote goes for punctured gut.
-vetticus3-
