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weird elisa result - (Sep/05/2009 )

i ran another commerical kit this morning, but the results were so weird. i did all my samples in triplicate, and some samples have very big standard deviation. for example, for same sample, one got 0.600 absorbance, the other two got 0.100 absorbance only. What happened? btw, my blank has higher absorbance than most of my samples, end up most of my samples have concentration of lower detection limit (< o ng/ml). Does this make sense?


No sense at all.
Check your equipment (washer,reader etc) carefully, it looks if there is something very wrong in your procedures.