Top : Molecular Biology : RNA : cDNA

Summary: cDNA stands for complementary DNA or copy DNA and can be single-stranded or double-stranded. cDNA is synthesized in vitro from a mRNA template using reverse transcriptase. The resulted cDNA is single-stranded. This process is called reverse transcription (RT) or first strand cDNA synthesis. In some experiments such as microarray, double-stranded cDNA is required and can be produced by another round of DNA synthesis following the first strand synthesis. The purpose of converting mRNA to cDNA is mainly for the analysis of the template mRNA because DNA is much stable than RNA. Once mRNA is converted to cDNA, the cDNA can be used for RT-PCR, as probe for expression analysis and for cloning of the mRNA sequence.
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