Top : Cell Biology : Stem Cells : Reprogramming of Somatic Cells into Stem (iPS) Cells

Summary: iPS cells or induced pluripotent stem cells are stem cell-like cells derived from reprogrammed somatic cells. iPS cells reprogramming is achieved by simultaneously expressing 2-4 ES cell specific transcriptional factors including Oct4, Sox2, Myc and Klf4/Lin28 using viral vectors (lentivirus or retrovirus). The reprogramming process takes 15-30 days. First, somatic cells such as fibroblast cells are infected with a mixture of viral particles expressing the 2-4 stem cell factors. The infected cells are then seeded on mouse embryonic fibroblast (MEF) feeder cells and cultured in ES cell medium. After 10-15 days, ES like colonies of cells will appear, can be picked and expanded. Reprogramming can also be done without using MEF feeder cells.
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