accidentally used denatured ethanol for DNA and RNA - what to do?
Posted 01 June 2013 - 12:55 PM
I added denatured ethanol to all the necessary buffers, then only I came to realize that we should use non-denatured ethanol for molecular biology applications because denatured ethanol contains impurities.
What should I do now?
Can I proceed with DNA and RNA isolation using the reagents?
If not, my supervisor is going to kill me OMG!!!
Posted 01 June 2013 - 02:26 PM
- jamestoon1 likes this
Posted 02 June 2013 - 01:01 PM
A single lie is reproachable; a million lies is a statistic.
D. J. T.
Posted 02 June 2013 - 04:59 PM
Posted 02 June 2013 - 08:36 PM
Due to shortage of time (I need to do some trial tests today), I proceeded with DNA and RNA isolation with the reagents added with denatured ethanol.
The spectrophotometric readings were good - the yield and purity were comparable to DNA/RNA isolated with the reagents with non-denatured ethanol.
Let's hope any impurities that may be present would not interfere with downstream applications.