Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Black moving particles in the cell Culture

Bacteria

  • Please log in to reply
2 replies to this topic

#1 Mati

Mati

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 30 posts
0
Neutral

Posted 02 December 2011 - 04:11 AM

Hi guys i am using HEK 293 cells but i found some black particles moving in my cell culture even some are attached to the surface of the cells.
Can i use these cells for transfection??? I have attached some pics of these particles plz let me know if you have soem idea??

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,724 posts
568
Excellent

Posted 02 December 2011 - 02:17 PM

See here: http://www.protocol-...-helpful-paper/

#3 Gangwolf

Gangwolf

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 41 posts
1
Neutral

Posted 22 December 2011 - 02:29 AM

Take the supernatant containing the black dots and "culture" this in a cell culture incubator separately from your eukaryotic cells. If these dots are proliferating then I would say it is bacterial contamination.
Upon any bacterial contamination, discard your contaminated cells as they would give you inconsistant experimental results. In addition, contaminated cells might be very difficult to transfect.

Good luck





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.