Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Quantifying DNA from Buccal Swabs

DNA quantification buccal swabs

  • Please log in to reply
1 reply to this topic

#1 ca.dreaming



  • Members
  • Pip
  • 1 posts

Posted 13 October 2011 - 04:57 PM

So I'm collecting DNA from Buccal Swabs but having some trouble quantifying it. Is there any DNA extraction kit that allows the use of Nanodrop???
Otherwise, Im trying to create a standard curve for fluorescence using Hoechst Dye. Right now I've got DNA concentrations between 0 and 2.6 micrograms/ml and 1 mg/ml Hoechst Dye in a 100microliter well with buffer.
352nm excitation, 461nm emission wavelengths...

But my values are all over the board and not linear at all.
Any idea where I could be going wrong?

Any help would be much appreciated!

#2 phage434



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,747 posts

Posted 14 October 2011 - 06:44 AM

I'd make sure my quantitation was working by testing serial dilutions of a known DNA concentration. Those concentrations sound very low, and are likely near the limit of detection, but a serial dilution test will tell you if that is true.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.