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Photometric and fluid in the well

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#1 rock_scientist



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Posted 13 July 2011 - 07:14 AM

I am new to the proliferation assays.I have to perform the proliferation assay with 150uL of my drug and control according to the protocol.When I am done adding everything to the wells,the total amount of fluid is above 250uL before I perform the photometric reading.I wanted to know if this in any way would effect my absorbance values?

I will appreciate your help.


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