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MTT Assay Query

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#1 Meera



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Posted 01 June 2011 - 10:05 PM


Please could any one explain me that inorder to determine the OD in MTT assay. I have to take two readings at 560 nm and 670 nm and then subtract so to get the OD as it says "Read optical density at 560nm and subtract background at 670nm"

help please
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