Archived Protocol

<BODY BACKGROUND="../gif/chalk.gif" TEXT=00009C LINK=8E236B VLINK=CC3299 ALINK=FF1CAE>  <HTML> <title>Collection and Storage of Cotton Leaf Tissue</title> <HEAD> <DL><DD> <h2>Collection and Storage of Cotton Leaf Tissue</h2> </HEAD> <BODY> <ol> <li> Harvest cotton tissue of interest. Separate the different leaf sample in a plastic bag. <P> <li> Chill the samples on ice for transport back to the laboratory. <P> <li> Remove the midribs, if desired, then put the each sample in a 50 ml polypropylene tube (PPT). <P> <li> Place the samples into -70 C until freeze-drying them. <P> <li> Freeze-dry (See Lyophilization) the sample for 2 to 4 days. <P> <li> Store the freeze-dried leaf tissue in a 50 ml PPT tightly-capped at -20 C or in a desiccator at room temperature. </ol> </DL> </BODY>  </HTML>