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Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible)</td> </tr> <tr bgcolor="#EBEBEB" class="style15"> <td colspan="2">2. Add the same volume of a 1M KCl, 0.2M Phosphoric acid solution and vortex again</td> </tr> <tr bgcolor="#EBEBEB" class="style15"> <td colspan="2">3. Centrifuge sample at 10,000 xg for 2 minutes</td> </tr> <tr bgcolor="#EBEBEB" class="style15"> <td colspan="2"><P>4. Collect the bottom phase (contains lipids)</P></td> </tr> <tr class="style15"> <td> </td> <td> </td> </tr> </table> </div> <P class="style15"> </P> <P class="style15"> </P> <P class="style15"> </P> <P class="style15"> </P> <P class="style15"> </P> </BODY> </HTML>