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   ChIP on Chip

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Contact:
Lee Edsall
ledsall@ucsd.edu

Protocols used in ChIP-on-chip experiments

Listed here are collections of RenLab protocols as well as protocals requested from other labs.

  1. Solutions
  2. Cross-linking of cells and fragmentation and immunoprecipitation of chromatin
  3. Blunting, ligation of linkers to DNA, and amplification by PCR
  4. Labeling of DNA and microarray hybridization
  5. Microarray analysis and identification of in vivo DNA binding sites