|ChangBioscience||Primo Pro 3.4: PCR Primer Design           User Instructions FAQ Order Home|
|Primo Pro 3.4 reduces PCR noise by lowering the probability of random primering. For example ACTACCTGCTGCTGCAC for the p53 gene looks OK because it meets criteria commonly used for primer design. This primer may have high background because the 3' 8 nucleotides appears in 4800 genes in human transcriptome. Primo Pro analyzed transcriptomes to mark sequences that are over-represented, thus allow users to select primers with few over-represented sequences at the 3'-end. As a result of reduced random priming, we expect improved amplification efficiency and cleaner products, especially for RT-PCR. Order Primo Pro New: batch mode. See also Primo Family.|
|since 6/1/02.||Warning! Uncheck "Avoid background priming" if PCR template is not human cDNA. Read user manuel for details.|
| Primo Online runs on Java-enabled browsers|
PC: Internet Explorer 5.5 or higher and Netscape 4.08 or higher
Mac: Internet Explorer 5.0, IE5.1.4 or higher (IE5.1 will not work because of a bug.)
| Need to raise a better antibody? |
Please use online Abie Pro 3.0 for peptide antibody design.
Pro --- Standard PCR and RT-PCR.
Multiplex --- Multiple PCRs in one tube.
Degenerate --- Degenerate PCR for one or more genes.
Optimum --- Optimize codon usage. Gene Synthesis.
Diverse --- Most diverse regions of a family.
Unique --- Amplify one gene from a family.
MSP --- Methylation specific PCR.
Random --- Random primers for fingerprinting.
Profile --- Profile 10s-1000s of genes.
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