| MS Plant Tissue Culture Medium | ||
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| Reference: Murashige and Skoog (1962) | Last updated: 2/16/00 | By: Kay Schneitz |
You can also buy the various MS solutions from e.g. Phytotechnology Laboratories.
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| Component | mg/l in MS | mg/l in stock | Amount for |
| MS Macro I | use as 10x stock | 1 liter | |
| NH4NO3 | 1650 | 16.500 | 16.5 g |
| KNO3 | 1900 | 19.000 | 19.0 g |
| MgSO4.7H2O | 370.6 | 3706 | 3.706 g |
| KH2PO4 | 170 | 1700 | |
| 100 ml/l | |||
| MS Macro II | use as 10x stock | 1 liter | |
| CaCl2.H2O | 439.8 | 4398 | 4.398 g |
| (100 ml/l) | |||
| FeNaEDTA | use as 100x stock | 100 ml | |
| 100 ml/l | 36.7 | 3670 | 367 g |
| MS Micros | use as 1000x stock | 100 ml | |
| H3BO3 | 6.20 | 6200 | 620 mg |
| MnSO4.2H2O | 13.2 | 13200 | 1320 mg |
| ZnSO4.7H2O | 8.6 | 8600 | 860 mg |
| Na2MoO4.2H2O | 0.25 | 250 | 25 mg |
| CuSO4.5H2O | 0.025 | 25 | 2.5 mg |
| CoCl2.2H2O | 0.025 | 25 | 2.5 mg |
| KI | 0.83 | 830 | 83 mg |
| 1 ml/l | |||
| Myo-inositol | use as 100x stock | 100 ml | |
| 100 | 10000 | 1 g | |
| 10 ml/l | |||
| MS Vitamines | use as 1000x stock | 100 ml | |
| Nicotinic acid | 0.5 | 500 | 50 mg |
| Pyridoxine-HCl | 0.5 | 500 | 50 mg |
| Thiamine-HCl | 0.1 | 100 | 10 mg |
| Glycine | 2 | 2000 | 200 mg |
| 1 ml/l | |||
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