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April 19 1990
Jim Howe
Purpose:
Time required:
Special Materials:
Procedure:
Day 1
Day 2
a. 10% SDS b. 0.5 N NaOH 1.5 M NaCl c. 1.5 M NaCl 0.5 M Tris-HCl pH 7.4 d. 2X SSC
Pour off any excess liquid for this will cause colonies to swell and blur subsequent hybridization signals.
References:
Sambrook J. Fritsch E.F. and T. Maniatis.(1989) Molecular Cloning A Laboratory Manual. Second edition. Cold Spring Harbor Laboratory Press pp. 1.90-1.100.