Protein Extraction from Plant
(The University of Nebraska-Lincoln Protein Core Facility ) A common protocol used for extraction proteins from plant tissues, consists in the homogenization of mortar-grounded material in liquid nitrogen with an extraction buffer (20 mM Tris-HCl, pH 8.0, 5 mM EDTA, 50 M leupeptin, 1 M pepstatin A, 10 M 3, 4-dichloroisocumarine, 1 mM phenylmethylsulfonyl fluoride and 0.05 % SDS)... http://www.biotech.unl.edu/proteomics/Mercaptoetha... Added: Tue Aug 13 2002, Hits: 5245, Reviews: 1 Read reviews
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