Water Treatment with DEPC

Author: Protocol Online

Source: Protocol Online
Abstract: Treat water with DEPC to eliminate RNase
Date Added: 2003-05-08
Date Modified: 2009-02-17
Add 0.1% diethylpyrocarbonate (DEPC) to water, mix overnight, and then autoclave 20 min to destroy DEPC by causing hydrolysis of DEPC.


Notes:
DEPC is highly toxic.
Protocol Online
http://www.protocol-online.org
© 1999-2009 Protocol Online, All rights reserved.