Stripping Western Membranes

Author: Monica Colitti

Source: Contributed by Monica Colitti
Abstract: Simple protocol for reusing blot
Date Added: 2002-05-14
Date Modified: 2004-04-29

Procedure

  1. Put the membrane in PBS or TBS with 7ul/ml B-mercaptoetanol and 2%SDS for 30' at RT in agitation 
  2. Wash the membrane for 30' in PBS or TBS.
  3. Put the membrane in blocking buffer

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