PCR Master Mix Calculator
Number of reactions:
Reaction volume:
µl
Allowance for error:
%
Magnesium sulphate in buffer?
Reagent
Stock Concentrations
Desired Values
Reaction Mix (µl)
Reaction buffer
X
X
Magnesium Sulphate
mM
mM
dNTPs (total)
mM
mM
Forward primer
µM
µM
Reverse primer
µM
µM
DNA template
ng/µl
ng
Taq Polymerase
U/µl
U
Water
Total reaction volume:
µl