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Lab break - (Nov/19/2006 )

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QUOTE (perneseblue @ Feb 11 2007, 09:26 AM)
QUOTE (krümelmonster @ Feb 9 2007, 05:13 PM)
... I don't even want to know what you used to replace the creamer????

biggrin.gif

no doubt it was the blocking solution from western blots

So tell us, what kind of flavour does BSA give to coffee? biggrin.gif biggrin.gif biggrin.gif wacko.gif

-swanny-

I figure if most reagents are pure enough to use in vitro, its good enough for in vivo.

For creamer you would use Carnation non fat dry milk bought from the grocer. Not BSA.

-pBluescript-

Power shake for Scientist tongue.gif

1 L
10g/L BSA
10mM Glucose
50mM Glutamate
5mM Sodium chloride
1%v/v Glycerol
0.05% v/v Palmatic acid

Make up in 1L bottle
Autoclave

Add 5ml 100% Ethanol (optional)

Place in beveled flask and shake for 1hr in shaker
Cool on ice for 30min.
Aliquote in 50ml tube and introduce orally.

-perneseblue-

QUOTE (perneseblue @ Apr 16 2007, 01:07 PM)
Power shake for Scientist tongue.gif

1 L
10g/L BSA
10mM Glucose
50mM Glutamate
5mM Sodium chloride
1%v/v Glycerol
0.05% v/v Palmatic acid

Make up in 1L bottle
Autoclave

Add 5ml 100% Ethanol (optional)

Place in beveled flask and shake for 1hr in shaker
Cool on ice for 30min.
Aliquote in 50ml tube and introduce orally.


Hi perneseblue,

Have you try it?

-Minnie Mouse-

That recipe sounds absolutely revolting!

I don't care how good for you it may be!

-Cassio-

QUOTE (Minnie Mouse @ Apr 17 2007, 06:58 AM)
Hi perneseblue,

Have you try it?


thank goodness, no. Never touched it. But I would be most happy if some brave and fool hardy soul decided to give this recipie and protocol a go. It has body, salt, glutamate, and it is sweat too! wink.gif What more could anyone want?

-perneseblue-

I guess from the fact that he is able to begin this thread shows (or should i say "suggests") that he survived the lab glucose and blocking solution coffee.

-The_Erotic_Terrorist-

QUOTE (perneseblue @ Apr 16 2007, 05:07 PM)
Power shake for Scientist tongue.gif

1 L
10g/L BSA
10mM Glucose
50mM Glutamate
5mM Sodium chloride
1%v/v Glycerol
0.05% v/v Palmatic acid

Make up in 1L bottle
Autoclave

Add 5ml 100% Ethanol (optional)

Place in beveled flask and shake for 1hr in shaker
Cool on ice for 30min.
Aliquote in 50ml tube and introduce orally.


Only 5ml Ethanol per 1 liter of "shake"???? That doesn't seem quite right to me.

-JVBishop-

There is a rule about not to eat or drink in the lab... Only exception to that rule is EtOH. happy.gif

-DLY-

Remember to only drink the 95% EtOH. The absolute ethanol contains benzene, which is both bad for you and tastes bad.

-phage434-

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