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- Plant Molecular Biology Protocols (By John Mundy, hosted by The Arabidopsis Information Resource)
A large collection of protocols on plant work especially on arabidopsis. All on one page including these topics: Agrobacterium competent cells / Agrobacterium electroporation / Agrobacterium miniprep / Agrobacterium miniprep (phenol free) / Arabidopsis transformation (infiltration) / cDNA probes / Colony hybridization / Concanavalin A Blots / DNA PAGE / E coli competent cells (CaCl2) / E coli competent cells (RuCl2) / E coli transformation / End labelled probe preparation / Filter washing / Fluorography of PAG / Fluorometer/Luminometer Wallac Victor II / Footprint with DNAse1 / Gelshift / Genomic DNA from cereal leaves / GST fusion protein purification / GUS histochemical assay / GUS & LUC bombardment assay / GUS luminometric assay / HIS-tagged protein purification / Hormone treatments / Hormones & inhibitors / Hybridization selection of cloned DNA / Immunoprecipitation from in vitro translations / Isotachophoresis probe isolation / In vitro transcription / In vitro translation / Isolation of Lambda DNA by plate lysate / Lambda lysate / Leaf PCR / Maxam Gilbert sequencing / Microsomal preps for membrane proteins / Miniprep - alkaline lysis / NEpHGE Non-equilibrium pH gel electrophoresis / Northern blot in formaldehyde gel / Nuclear extract preparation / Nuclei prep / Nuclear prep from protoplasts / Oligo dT cellulose purification of mRNA / Oligo probe hybridization / Oligonucleotide probe preparation / Phage strains / Plasmid prep with CsCl / Primer extension with synthetic oligonucleotide / Progeny analysis of plants / RNA extraction in GT w/ CsCl spin / RNA large scale from starchy tissue / RNA miniprep 1 / RNA miniprep 2 / RT-PCR / SDS-PAGE for proteins / Seed sterilization for cereals / Silver staining of proteins in PAG / Soluble protein extraction / Southwestern blotting / T4 polymerase fill in rxn / Western blotting / Whole cell extracts /
http://www.arabidopsis.org/info/Protocols_Mundy2.h...
Added: Tue Jul 30 2002, Hits: 2185, Reviews: 0 Write review Cached -
AFLP: for positional cloning
(Department of Plant Biology, Stanford University)
AFLP was designed as a highly sensitive method for DNA fingerprinting to be used in a variety of fields, including plant and animal breeding, medical diagnostics, forensic analysis and microbial typing, etc. This protocol has been used to generate DNA based markers for cloning genes involved in phototropic responses in higher plants that have only been identified genetically by mutant phenotype(s)
http://www-ciwdpb.stanford.edu/publications/method...
Added: Tue Jul 30 2002, Hits: 649, Reviews: 0 Write review Cached -
CIMMYT Applied Molecular Genetics Laboratory Protocols (3rd Edition)
(CIMMYT Applied Molecular Genetics Laboratory)
A collection of protocols on studying genetics of maize and wheat.
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Sun Mar 22 2009, Hits: 902, Reviews: 0 Write review -
Complementation in Arabidopsis
(Preuss Lab, University of Chicago)
Complementation is performed to rescue the phenotype of a mutant plant with the cloned wildtype gene. This can prove that the gene cloned is in fact the gene that was mutated in the mutant plant.
http://preuss.bsd.uchicago.edu/protocols/complemen...
Added: Sat Apr 10 2010, Hits: 520, Reviews: 0 Write review -
Fixation of Plant Metaphase Chromosomes
(Pat Heslop-Harrison and Trude Schwarzacher, Department of Biology, University of Leicester)
http://www.le.ac.uk/bl/phh4/proaccum.htm
Added: Sat Mar 27 2004, Hits: 663, Reviews: 0 Write review Cached -
Histochemical assays for plant sections
(Atle M. Bones, The NTNU Plant Genetics Lab)
Some histochemical tests for fresh plant tissue clices
http://boneslab.bio.ntnu.no/old_root/histochemical...
Added: Thu Oct 10 2002, Hits: 624, Reviews: 0 Write review Cached -
Isolation of Retroelement from Plant Genomic DNA
(Pat Heslop-Harrison and Trude Schwarzacher, Department of Biology, University of Leicester)
Retroelements and their derivatives are an ubiquitous and abundant component of plant genomes. From the 1990s, PCR based techniques have been developed to isolate the elements from genomic DNA of different plants, and the methods and primers used are presented here.
http://www.le.ac.uk/biology/phh4/methods/retros.ht...
Added: Sat Mar 27 2004, Hits: 74, Reviews: 0 Write review Cached -
Lyophilization and Grinding of Leaf Tissue
(CIMMYT Applied Molecular Genetics Laboratory)
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Fri Aug 02 2002, Hits: 1302, Reviews: 0 Write review -
Lyophilization of cotton leaves
(Lazo Lab, USDA)
http://wheat.pw.usda.gov/~lazo/methods/lazo/lyophi...
Added: Sun Mar 22 2009, Hits: 362, Reviews: 0 Write review Cached -
Measurement of Carbon Fixation Rates in Leaf Samples
(David R. Caprette, Rice University )
Use of carbon-14 labeled sodium bicarbonate to estimate photosynthetic rates
http://www.ruf.rice.edu/%7Ebioslabs/studies/leaves...
Added: Sun Sep 29 2002, Hits: 113, Reviews: 0 Write review Cached -
Protein Extraction from Plant
(The University of Nebraska-Lincoln Protein Core Facility )
A common protocol used for extraction proteins from plant tissues, consists in the homogenization of mortar-grounded material in liquid nitrogen with an extraction buffer (20 mM Tris-HCl, pH 8.0, 5 mM EDTA, 50 M leupeptin, 1 M pepstatin A, 10 M 3, 4-dichloroisocumarine, 1 mM phenylmethylsulfonyl fluoride and 0.05 % SDS)...
http://www.biotech.unl.edu/proteomics/Mercaptoetha...
Added: Tue Aug 13 2002, Hits: 1884, Reviews: 1 Read reviews Write review Cached -
Sterilizing Seed
(Preuss Lab, University of Chicago)
http://preuss.bsd.uchicago.edu/protocols/sterl.htm...
Added: Sat Apr 10 2010, Hits: 600, Reviews: 0 Write review