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Protocols
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Platelet-associated Ig (PAIg) Elution
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Platelet-associated Ig (PAIg) could be eluted from the platelets. As a result of this procedure platelets get destroyed and the eluted Ig is formed of platelet surface-bound Ig and , mainly, of internal pools of Ig (from a-granules). That is why the caution must be taken when interpreting results.
http://www.icp.ucl.ac.be/mexp/vipg_plig.html
Added: Wed Mar 24 2004, Hits: 222, Reviews: 0 Write review Cached -
Preparation & Analysis of Cellular Suspensions of Bone Marrow of Rats and Mice
(Clinical Pathology Laboratory, National Institute of Environmental Health Sciences)
To evaluate bone marrow cellularity and morphology, a suspension of cells is prepared from the femur of a rat or mouse.
http://dir.niehs.nih.gov/dirlep/Webpages/techniq.h...
Added: Thu Jul 13 2006, Hits: 1627, Reviews: 0 Write review Cached -
Serum Separation from Whole Blood
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Method for obtaining serum from clotted blood
http://www.nottingham.ac.uk/~mbzspd/methods/Serum_...
Added: Sat Apr 10 2004, Hits: 1341, Reviews: 0 Write review Cached -
Spectrophotometric Methemoglobin Analysis
(Clinical Pathology Laboratory, National Institute of Environmental Health Sciences)
A hemolysate is prepared from a blood sample by addition of water and phosphate buffer. Two equal portions of hemolysate are pipetted into cuvettes...
http://dir.niehs.nih.gov/dirlep/Webpages/techniq.h...
Added: Thu Jul 13 2006, Hits: 387, Reviews: 0 Write review Cached -
Thrombophagocytosis
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
This protocol represents an Ab-mediated thrombophagocytosis modification. Well written with illustration.
http://www.icp.ucl.ac.be/mexp/vipg_thrph.html
Added: Sun Mar 21 2004, Hits: 365, Reviews: 0 Write review Cached -
Western Blot with Platelet Protein
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Detailed western protocol devoted to detecting paltelet protein but can also be used for general purpose.
http://www.icp.ucl.ac.be/mexp/vipg_plwb.html
Added: Mon Mar 22 2004, Hits: 4029, Reviews: 0 Write review Cached