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Protocols
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Bleeding Time Test
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
http://www.icp.ucl.ac.be/mexp/vipg_blt.html
Added: Sun Mar 21 2004, Hits: 1051, Reviews: 0 Write review Cached -
Blood Smear Preparation and Staining
(The University of Texas Medical School,Department of Pathology and Laboratory Medicine)
A thin blood smear is stained with Wright-Giemsa stain to enable evaluation of white blood cell, red cell and platelet morphology.
http://medic.med.uth.tmc.edu/path/00000288.htm
Added: Sun Mar 21 2004, Hits: 248, Reviews: 0 Write review Cached -
Clot Retraction Test
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Abnormal Clot Retraction indicates on abnormal platelet numbers, abnormal glycoprotein amount or structure, abnormal platelet signaling, abnormal fibrinogen levels, or abnormal fibrinogen structure
http://www.icp.ucl.ac.be/mexp/vipg_clret.html
Added: Sun Mar 21 2004, Hits: 842, Reviews: 0 Write review Cached -
CMFDA Labeling of Platelet
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
CMFDA (5-chloromethylfluorescein diacetate) is a lipophilic tracer that has an enormous advantage over ordinary tracers (e.g. FITC) because it can be introduced into live (!) cells. Once inside the cell this dye undergo some covalent changes restricting from its passgae to extracellular milieu.
http://www.icp.ucl.ac.be/mexp/vipg_plcmfda.html
Added: Wed Mar 24 2004, Hits: 817, Reviews: 0 Write review Cached -
Coating of Platelets with Antibody in vitro
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Antibody-coated platelets (opsnized) may be used in the subsequent thrombophagocytosis assay.
http://www.icp.ucl.ac.be/mexp/vipg_plab.html
Added: Wed Mar 24 2004, Hits: 709, Reviews: 0 Write review Cached -
Cytology of Mammalian Blood
(David R. Caprette, Rice University )
preparation of blood smear and microscopic examination of whole blood for identification of formed elements
http://www.ruf.rice.edu/%7Ebioslabs/studies/sds-pa...
Added: Sun Sep 29 2002, Hits: 1386, Reviews: 0 Write review Cached -
ELISA with Platelet
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg)
http://www.icp.ucl.ac.be/mexp/vipg_plelisa.html
Added: Wed Mar 24 2004, Hits: 1611, Reviews: 0 Write review Cached -
Erythrophagocytosis
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
The erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts.
http://www.icp.ucl.ac.be/mexp/vipg_eph.html
Added: Fri Mar 26 2004, Hits: 1190, Reviews: 0 Write review Cached -
Platelet Preparation
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
This protocol is based on differential centrifugation that allows to separate platelets from blood cells.
http://www.icp.ucl.ac.be/mexp/vipg_plpre.html
Added: Sun Mar 21 2004, Hits: 2434, Reviews: 0 Write review Cached -
Platelet-associated Ig (PAIg) Elution
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Platelet-associated Ig (PAIg) could be eluted from the platelets. As a result of this procedure platelets get destroyed and the eluted Ig is formed of platelet surface-bound Ig and , mainly, of internal pools of Ig (from a-granules). That is why the caution must be taken when interpreting results.
http://www.icp.ucl.ac.be/mexp/vipg_plig.html
Added: Wed Mar 24 2004, Hits: 674, Reviews: 0 Write review Cached -
Serum Separation from Whole Blood
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Method for obtaining serum from clotted blood
http://www.nottingham.ac.uk/~mbzspd/methods/Serum_...
Added: Sat Apr 10 2004, Hits: 186, Reviews: 0 Write review Cached -
Thrombophagocytosis
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
This protocol represents an Ab-mediated thrombophagocytosis modification. Well written with illustration.
http://www.icp.ucl.ac.be/mexp/vipg_thrph.html
Added: Sun Mar 21 2004, Hits: 731, Reviews: 0 Write review Cached -
Western Blot with Platelet Protein
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Detailed western protocol devoted to detecting paltelet protein but can also be used for general purpose.
http://www.icp.ucl.ac.be/mexp/vipg_plwb.html
Added: Mon Mar 22 2004, Hits: 2181, Reviews: 0 Write review Cached