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FAQs for Antibiotics
(Invitrogen)
tips for does and preparation of Geneticin (G418)
http://invitrogen.custhelp.com/cgi-bin/invitrogen....
Added: Tue May 14 2002, Hits: 1420, Reviews: 0 Write review Cached -
Generation of recombinant baculoviruses by co-transfection
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
For co-transfection prepare at least 10mg of highly purified plasmid DNA. Spodoptera frugiperda cells are sensitive to some contaminants found in crude plasmid preparations, which cannot be removed by phenol/chloroform extraction or ethanol precipitation.
http://www.its.caltech.edu/%7Ebjorker/Baculovirus-...
Added: Wed Aug 14 2002, Hits: 1781, Reviews: 0 Write review -
Optimize DNA Transfection
(Mirus Bio, LLC)
Tips on optimizing conditions for DNA transfections
http://www.mirusbio.com/tech_resources/tips/optimi...
Added: Tue Mar 12 2013, Hits: 61, Reviews: 0 Write review -
Selection of Transfected Suspension Cells
(Hannun/Obeid Lab)
http://www.musc.edu/bcmb/ceramide/protocols/0008.h...
Added: Tue May 14 2002, Hits: 1572, Reviews: 0 Write review Cached -
Transfection normalizing using dot-blot hybridisation
(Methods-Online)
It is a known, that the usage of plasmid expressing reporter gene driven by strong promoter causes problems when one wants to compare different cell lines. This method allowes you to measure directly the amount of the plasmid which entered the cells and therefore provides a reliable reference.
http://www.methods.info/Methods/Transfection/Trans...
Added: Wed Sep 04 2002, Hits: 719, Reviews: 0 Write review Cached -
Transfection of Mammalian Cells Using Lipofectamine (Invitrogen)
(Julie B. Wolf, University of Maryland, Baltimore County)
http://www.research.umbc.edu/~jwolf/m10.htm
Added: Tue May 14 2002, Hits: 5197, Reviews: 0 Write review Cached -
Transfection with Liposomes
(Bioinformatics Courses at the Bioinformatics Centre, National University of Singapore)
Transfection of mammalian cell lines is usually accomplished with calcium phosphate or liposomes. Transfection by liposomes is rapid and has high efficiency, but is more expensive. The procedure is very simple in essence, but there are a large number of parameters which can be tweaked, including ratio of DNA to liposomes, amount of DNA per well, time of incubation etc.
http://everest.bic.nus.edu.sg/hons99/gekming/proto...
Added: Sat Feb 16 2013, Hits: 50, Reviews: 0 Write review Cached -
Transient transfection
(Springer Lab, Harvard Medical School)
CaCl2 method
http://labs.idi.harvard.edu/springer/uploads/Proto...
Added: Tue Aug 13 2002, Hits: 1964, Reviews: 0 Write review Cached -
Transient Transfection of Cos-1 Cells
(Upstate)
This protocol has been optimized for Cos-1 cells. Successful transfection of each cell type requires optimization of the basic protocol. Variables to consider for optimization include, but are not limited to, cell density, transfection reagent, duration of transfection, and DNA concentration.
http://www.upstate.com/misc/protocols.q.prot.e.cDN...
Added: Tue Feb 24 2004, Hits: 2144, Reviews: 0 Write review Cached