Top : Molecular Biology : RNA : microRNA : microRNA (miRNA) and Small RNA Isolation, Extraction and Purification
- Isolation of micro-RNA (miRNA) (Protocol Online)
This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including small RNAs. The conditions for extraction enable the partitioning of proteins and DNA into the organic layer of the biphasic solution interface, while retaining RNA in the upper aqueous layer. The aqueous phase is removed to a second tube, and RNA is precipitated with an equal volume of isopropanol. High yields of pure, undegraded total RNA can be recovered from even small quantities of tissue or cells. Large numbers of samples may be performed simultaneously, because of the simplicity of the technique.
Added: Sat Jan 31 2009, Reviews: 0
Gel Purification of miRNA from Total RNA
Describes a method for purifying miRNAs from total RNA using PAGE gel.
Added: Sat Jan 31 2009, Hits: 2848, Reviews: 0
microRNA Extraction and Purification
(Microarray Core Facility, UT Southwestern Medical Center at Dallas)
This microRNA extraction protocol is part of a microRNA microarray protocol and consists of three major parts: cell lysis and tissue disruption, organic extraction and enrichment procedure for small RNAs.
Added: Sat Jan 31 2009, Hits: 3208, Reviews: 0