Protocols

• DNase I Treatment Of Total RNA (Microarray Core Facility, Wadsworth Center)
  This protocol involves treating RNA with DNase I and stopping the reaction with EDTA followed by purifying RNA with Qiagene RNeasy kit.
  http://www.wadsworth.org/genomics/microarray/total...
  Added: Wed Nov 23 2005, Hits: 954, Reviews: 0  Write review   Cached
• DNase Treatment (Practical Approach Online, Oxford University Press)
  DNase treatment of total cellular RNA to remove contaminating genomic DNA
  http://www.oup.co.uk/pdf/pas/5-7-4.pdf
  Added: Sat Aug 17 2002, Hits: 4006, Reviews: 0  Write review  
• How can I treat my RNA sample before RT-PCR to eliminate DNA contamination? (Invitrogen)
  http://invitrogen.custhelp.com/cgi-bin/invitrogen....
  Added: Tue May 14 2002, Hits: 6264, Reviews: 0  Write review   Cached
• Methods to Remove DNA Contamination from RNA Samples (Ambion)
  various methods for removing DNA contamination
  http://www.ambion.com/techlib/tb/tb_181.html
  Added: Wed Jul 07 2004, Hits: 3710, Reviews: 0  Write review   Cached

Trouble Shootings and FAQs

• Tips on DNase treatment for purfcation of RNA
• After adding the isopropyl alcohol, some fibrils-like material appears
• How to get rid of DNA contamination in RNA isolation