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Protocols
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Immunoblotting (Western Blotting)
(Sigma-Aldrich Corporation)
The electrophoretic transfer of proteins from sodium dodecyl sulphate polyacrylamide gels (SDS-PAGE) to sheets of nitrocellulose was initially referred to as "Western" blotting or Immunoblotting.
http://www.sigmaaldrich.com/Area_of_Interest/Life_...
Added: Fri Jul 11 2008, Hits: 150, Reviews: 0 Write review -
Immunoblotting Protocol
(eBioscience)
Immunoblotting technique provides information about presence, molecular weight, and/or quantity of an antigen by combining protein separation via gel electrophoresis with specific recognition of antigens by antibodies. This protocol offers general guidelines and procedure for immunoblotting.
http://www.ebioscience.com/ebioscience/appls/WB.ht...
Added: Mon Jan 02 2006, Hits: 5528, Reviews: 0 Write review Cached -
MAP Kinase Gel Shift Assay
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 1022, Reviews: 0 Write review Cached -
Preparation of Brain Membrane Fractions for Western Blot Analysis
(BD PharMingen)
This is a standard protocol adapted from Rogers et al., 1991.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Tue May 14 2002, Hits: 2940, Reviews: 0 Write review Cached -
Preparing Protein Lysates for Western Blotting
(Antisense Research Group)
http://www.liv.ac.uk/~giles/methods/western_lysate...
Added: Tue May 14 2002, Hits: 3833, Reviews: 0 Write review Cached -
Western Blot Protocol
(South African Structural Biology Network)
Brief introduction, references, reagents and step-by-step procedure for Transfer of protein to the membrane, Immunoassay (Chromogenic blots and Enhanced Chemiluminescence [ECL])
http://sbio.uct.ac.za/Sbio/documentation/western_b...
Added: Wed Aug 01 2007, Hits: 3298, Reviews: 0 Write review Cached -
Western Blot with Platelet Protein
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Detailed western protocol devoted to detecting paltelet protein but can also be used for general purpose.
http://www.icp.ucl.ac.be/mexp/vipg_plwb.html
Added: Mon Mar 22 2004, Hits: 4043, Reviews: 0 Write review Cached -
Western Blots
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt4/ex4-8...
Added: Tue May 14 2002, Hits: 10309, Reviews: 0 Write review Cached -
Western Blotting
(Howell Lab)
Procedures for preparation of cell lysate, gel, electrophoresis, transfer, antibody detection and stripping membranes.
http://cancer.ucsd.edu/howelllab/Western.html
Added: Tue May 14 2002, Hits: 22527, Reviews: 0 Write review Cached -
Western Blotting
(Corbett Lab)
http://www.biochem.emory.edu/labs/acorbe2/protocol...
Added: Tue May 14 2002, Hits: 6253, Reviews: 0 Write review Cached