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Protocols
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Bacculovirus Production : for Protein Expression
(Mike A. Dyer)
This is a modified version of the pharmingen Baculogold kit
http://axon.med.harvard.edu/~cepko/protocol/mike/B...
Added: Tue May 14 2002, Hits: 1126, Reviews: 0 Write review Cached -
Bacterial Expression of GST-fusion Proteins
(Whitelabs.org)
http://www.whitelabs.org/Lab%20Protocols/bacterial...
Added: Fri Nov 06 2009, Hits: 1566, Reviews: 0 Write review Cached -
Bacterial Expression of IRS-1 containing GST-fusion Proteins
(Whitelabs.org)
http://www.whitelabs.org/Lab%20Protocols/bacterial...
Added: Fri Nov 06 2009, Hits: 458, Reviews: 0 Write review Cached -
High cell-density fermentation of Escherichia coli
(Dr. Arie Geerlof)
This is a 5-day protocol for high cell-density fermentation of E. coli strains and overnight over-expression of (a) target protein(s) using a computer controlled fed-batch procedure.
http://www.embl-hamburg.de/~geerlof/protocols/HCDF...
Added: Sun Oct 25 2009, Hits: 897, Reviews: 0 Write review - Protein Expression (Contributed by Mark's Lab)
Added: Tue May 14 2002, Reviews: 0 Write review -
Protein expression in minimal media
(Chen)
Protocol with application notes.
http://www.biochem.ucl.ac.uk/~chen/protocols/prote...
Added: Tue May 14 2002, Hits: 1866, Reviews: 0 Write review -
Protein expression in Mycobacterium smegmatis using the inducible acetamidase promoter
(Dr. Arie Geerlof)
http://www.embl-hamburg.de/~geerlof/protocols/Msme...
Added: Sun Oct 25 2009, Hits: 349, Reviews: 0 Write review -
Protein Expression in Pichia
(Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
Pichia pastoris is a methylotropic yeast used to express high amounts of protein. Secreted expression is achieved with a cleavalable faktor. To yield high expression strains it is important to select for multiple copies of the insert in the pichia genome.
http://www.pharma.ethz.ch/institute_groups/biomacr...
Added: Sun Mar 01 2009, Hits: 1065, Reviews: 0 Write review Cached -
Protein refolding
(Chen)
Proteins expressed in E. coli are often found as insoluble aggregates in inclusion body. It may be possible to increase the solubility for some porteins by reducing incubating temperature during protein expression, use of fusion partners such as GST, thioredoxin and maltose-binding protein, co-expression of chaperones etc.
http://www.biochem.ucl.ac.uk/~chen/protocols/refol...
Added: Tue May 14 2002, Hits: 2673, Reviews: 1 Read reviews Write review
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