Summary: RNA ChIP is a method to examine RNA-protein interactions. RNA ChIP is performed in a similar way as regular ChIP assay with a extra step of adding RNase inhibitor to protect RNA after cross-linking. The pull-downed RNA is detected by RT reaction followed by RT-PCR. High-throughput assay of the RNA is also possible such as ChIP sequencing.
- RNA ChIP Assay (Epigenome Network of Excellence)
In RNA-ChIP, RNA-protein interactions are fixed by reversible chemical cross-linking with formaldehyde followed by immunoprecipitation with antibodies against the candidate protein(s). RNAs that are associated with the protein are detected by reverse transcriptase-PCR (RT-PCR). The following procedure was used to examine protein-RNA interactions in mouse embryonic stem cells, but can be modified for other cell types.
Added: Sat Jan 24 2009, Hits: 1459, Reviews: 0
RNA ChIP Assay for Detecting DNA Bound siRNA
(Epigenome Network of Excellence)
RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications
Added: Sat Jan 31 2009, Hits: 666, Reviews: 0