Protocols
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A rapid micro chromatin immunoprecipitation assay (microChIP)
(Collas Lab, University of Oslo, Institute of Basic Medical Sciences)
micro ChIP protocol is suitable for up to eight parallel histone and/or transcription factor immunoprecipitations from a single batch of 1,000 cells. microChIP technique is also suitable for monitoring the association of one protein with multiple genomic sites in 100 cells.
http://www.collaslab.com/UserFiles/File/Dahl%20200...
Added: Tue Jan 27 2009, Hits: 2086, Reviews: 0 Write review -
Genome-Scale micro-ChIP-chip Using 10,000 Cells
(BioTechniques)
This miniaturized ChIP protocol using 10,000 cells (without the addition of carrier reagents) can be used to obtain enough sample material to analyze the entire human genome.
http://www.biotechniques.com/default.asp?page=aop&...
Added: Tue Jan 27 2009, Hits: 1463, Reviews: 0 Write review Cached -
MicroChIP Protocol
(Genome Center, UC Davis)
Protocol developed in Farnham lab for performing ChIP on very small cell numbers, as few as 10,000 cells.
http://www.genomecenter.ucdavis.edu/expression_ana...
Added: Tue Jan 27 2009, Hits: 1486, Reviews: 0 Write review