Top : Molecular Biology : Protein : Immunoprecipitation (IP) : Chromatin Immunoprecipitation (ChIP Assay) : ChIP on Chip

Summary: ChIP-on-chip (chromatin immunoprecipitation-on-chip), also known as Location Analysis (LA), is a high throughput (genome-wide) identification and analysis of DNA fragments that are bound by specific proteins such as histones, transcriptional factors. ChIP-on-chip invovles the following procedures: 1) crosslinking DNA with proteins; 2) sonication of DNA into small pieces; 3) immunoprecipitation of DNA-bound proteins with an antibody; 4) purification of DNA; 5)hybridization of DNA with DNA microarrys.