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Protocols
- Immunoprecipitation (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Immunoprecipitation is a technique that permits the purification of specific proteins for which a antibody has been raised. This primary antibody is either already bound to agarose or can be bound to the protein A/agarose beads during the procedure in order to physically separate the antibody-antigen complex from the remaining sample. Two procedures are described including reagents used.
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Added: Sat Nov 02 2002, Hits: 2961, Reviews: 0
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Cached - Immunoprecipitation of Proteins (Upstate)
Well written protocol from Upstate for general use.
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Added: Tue Feb 24 2004, Hits: 2843, Reviews: 0
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Cached - Metabolic labeling & Immunoprecipitation (Springer Lab, Harvard University)
Includes radiolabeling and immunoprecipitation in great details.
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Added: Tue Aug 13 2002, Hits: 1115, Reviews: 0
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Analysis of Protein by Immunoprecipitatioin
(P.J. Hansen Lab)
This protocol describes two methods (protein A and G) in great details as well as the principal, application and trouble shooting of immunoprecipitation.
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Added: Tue May 14 2002, Hits: 2569, Reviews: 1 Read reviews
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Cached - Antibody Precipitation with Protein A or G (Contributed by James Turmo)
Using Immobilized Protein A or Protein G for Immunoprecipitation.
Added: Tue May 14 2002, Reviews: 0
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Co-Immunoprecipitation Analysis of Protein-Protein Interaction
(Morrisey Lab, University of Pennsylvania)
This protocol uses total cell extracts to analyze putative protein-protein interactions in eukaryotic cells. One can also use nuclear extracts as a source for this protocol.
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Added: Mon Oct 14 2002, Hits: 2023, Reviews: 0
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Cold Immunoprecipitations
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
For screening soluble FCRN mutant transfected cell supernatants
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Added: Wed Aug 14 2002, Hits: 586, Reviews: 0
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General Principles of Immunoprecipitation
(Sefton Lab, Salk Institute)
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Added: Tue Aug 13 2002, Hits: 2081, Reviews: 0
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Cached - Glycolipid Binding Assay (Contributed by Pingsunjim, Paller’s Lab)
This protocol can be used for the detection of glycolipids binding to immunoprecipited protein.
Added: Tue May 14 2002, Reviews: 0
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Immunoprecipitation
(Rosen Lab, Department of Molecular and Cellular Biology, Baylor College of Medicine)
Formaldehyde cross-linking and chromatin immunoprecipitation assays of tissue culture cells
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Added: Sat Aug 24 2002, Hits: 1371, Reviews: 0
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Immunoprecipitation
(Hancock Lab)
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Added: Tue May 14 2002, Hits: 1411, Reviews: 0
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Immunoprecipitation and Immune Complex kinase assay
(Sefton Lab, Salk Institute)
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Added: Tue May 14 2002, Hits: 953, Reviews: 0
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Immunoprecipitation from Eukaryotic Cell Extracts
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
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Added: Tue May 14 2002, Hits: 1087, Reviews: 0
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Immunoprecipitation of 35S Labeled Cells
(BD PharMingen)
This protocol is for general use
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Added: Wed Aug 28 2002, Hits: 351, Reviews: 0
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Immunoprecipitation of metabolically labeled cells
(Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research )
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Added: Tue May 14 2002, Hits: 282, Reviews: 0
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Preparation of Modifed Radioimmunoprecipitation (RIPA) Buffer
(Upstate)
Detailed receipes and procedures.
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Added: Tue Feb 24 2004, Hits: 303, Reviews: 0
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