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Preparation of Site-Specific Histone Antibodies and Affinity Purifications
(Chromatin and Gene Expression Group, The University of Birmingham, UK)
Provides guidelines for preparation of polyclonal antibodies against histones, including steps from synthesis of N-terminal peptides of histones H2A, H2B, H3 and H4, conjugation of synthetic peptides to ovalbumin, immunization of rabbits, evaluation of the antibody response to affinity chromatography.
http://medweb.bham.ac.uk/research/chromatin/protoc...
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