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Protocols
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Antibody Purification using Protein A, Protein G, or Protein L Agarose
(Sigma-Aldrich Corporation)
This protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants using protein A, protein G, or protein L agarose.
http://www.sigmaaldrich.com/Area_of_Interest/Life_...
Added: Fri Jul 11 2008, Hits: 75, Reviews: 0 Write review -
Antibodyby Purification by Affinity Chromatography
(Mullins Lab, UCSF)
http://mullinslab.ucsf.edu/protocols/html/ANTIBODY...
Added: Mon Nov 15 2004, Hits: 2836, Reviews: 0 Write review Cached -
Column Purification of mAB
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
The FcRn column is usually used for "functional" purification of IgG's, i.e. by binding at pH 6 and eluting at pH 8. The procedure described here was developed to separate anti-FcRn antibodies from contaminating IgG's present in the ascites. Therefore binding is done at pH 8.5 to prevent the FcRn on the column from binding to the Fc portion of the IgGs in the sample; only IgG's specific for FcRn should be able to bind, as in a typical affinity purification. They are then eluted at pH 2.5.
http://www.its.caltech.edu/%7Ebjorker/Anti-FcRn_mA...
Added: Wed Aug 14 2002, Hits: 1802, Reviews: 0 Write review -
Coupling Antibodies to Protein A/G
(Breeden Lab)
http://www.fhcrc.org/labs/breeden/Methods/coupling...
Added: Tue May 14 2002, Hits: 1675, Reviews: 0 Write review Cached -
Coupling Peptides to Resin for Affinity Purification
(Mitshison Lab)
http://mitchison.med.harvard.edu/protocols/ab3.htm...
Added: Tue May 14 2002, Hits: 1883, Reviews: 0 Write review Cached -
General Protocol for Affinity Purification of Antibodies from Serum
(Cooper Lab, Department of Cell Biology & Physiology Washington University in St Louis)
This protocol requires an appropriate affinity column prepared using protein from native sources
http://www.cooperlab.wustl.edu/Methods/AffinPurifA...
Added: Mon Aug 14 2006, Hits: 731, Reviews: 0 Write review -
Making Affinity Columns
(XMMR)
http://www.xenbase.org/xmmr/Marker_pages/methods_p...
Added: Tue May 14 2002, Hits: 2072, Reviews: 0 Write review Cached -
Purification of Anti Peptide Antibody
(Mitshison Lab)
http://mitchison.med.harvard.edu/protocols/ab4.htm...
Added: Tue May 14 2002, Hits: 1515, Reviews: 0 Write review Cached -
Purification of mAb (IgG)
(Springer Lab, Harvard University)
Ammonium sulfate cut and purification of Abs by protein A column
http://www.cbrinstitute.org/investigators/springer...
Added: Wed Aug 14 2002, Hits: 1113, Reviews: 0 Write review Cached