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Protocols
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Archived Discussions and Troubleshootings on Promoter Experiments
(Protocol Online)
http://www.protocol-online.org/biology-forums/prom...
Added: Wed Feb 11 2009, Hits: 1298, Reviews: 0 Write review Cached -
Geneome Walking by PCR
(Siebert et al. NAR 1995)
http://www.pubmedcentral.nih.gov/articlerender.fcg...
Added: Wed Feb 11 2009, Hits: 1008, Reviews: 0 Write review Cached -
Genome walking by PCR
(Langdale Lab, Department of Plant Sciences, University of Oxford)
The protocol involves a nested PCR with a touch down program. This method is very prone to artefacts so you NEED to follow rigorously the requirements for the design of primers and the touch-down PCR program!
http://dps.plants.ox.ac.uk/langdalelab/protocols/P...
Added: Wed Feb 11 2009, Hits: 1824, Reviews: 0 Write review -
Genome walking using splinkerette PCR
(Cnidaria, University of California, Irvine)
An improvement on the vectorette approach is the so-called "splinkerette" method
http://www.ucihs.uci.edu/biochem/Steele/Splinkeret...
Added: Wed Feb 11 2009, Hits: 912, Reviews: 0 Write review