- Phage Titer (E.T. Wurtzel, Department of Biological Sciences, Lehman College)
Lambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clones. Large numbers of clones are often needed for construction of gene libraries that represent large populations of molecules. These libraries are also easy to sort through, as lambda phage clones are particularly easy to array spatially at high density. This is called plating a library. This protocol will plate phage at various densities in order to generate a titer (count) and amplify a library in order isolate DNA
Added: Mon Aug 26 2002, Hits: 956, Reviews: 0
(The Morimoto Lab, Northwestern University)
General methods of phage preparation and purification.
Added: Mon Mar 29 2004, Hits: 2758, Reviews: 0
Preparation of Phage Particles
(Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
Preparation of phage particles from phage vectors and from phagemids.
Added: Mon Sep 09 2002, Hits: 1682, Reviews: 0