Protocols
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PCR Guide
(LaboratoryExperiments.com)
Basic guide high school students, But very useful if you are new to molecular biology.
http://www.laboratoryexperiments.com/LAB2.htm
Added: Tue May 14 2002, Hits: 4547, Reviews: 0 Write review Cached -
PCR Program Design
(Octavian Henegariu)
For any given primer pair, the PCR program can be selected based on the composition (GC content) of the primers and the length of the expected PCR product. Here is a guide to select the cycling program of PCR.
http://info.med.yale.edu/genetics/ward/tavi/p08.ht...
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PCR Tips and Tricks
(The Genomic Variation Laboratory, University of California in Davis)
http://genome-lab.ucdavis.edu/Protocols/pcr_tips/p...
Added: Fri Aug 06 2004, Hits: 5868, Reviews: 0 Write review Cached -
Standard PCR
(Biotech)
Detailed PCR protocol including preparation of the sample, the master mix and the primers, detection and analysis of the reaction products.
http://www.accessexcellence.org/LC/SS/PS/PCR/PCR_t...
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Standard PCR
(Dr. Chen, Dept of Biochem. & Mol. Biology, Univ. College London)
Standard PCR, primer design, cycling, additives and more
http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/pro...
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Standard PCR Protocol
(Molecular Biology Techniques Manual)
The followings are described in detail
Recommended Reagent Concentrations
Recommended Reaction Conditions
"Hot Start" PCR
Asymmetric PCR for ssDNA Production
Detecting Products
Labeling PCR Products with Digoxigenin
Cleaning PCR Products
Sequencing PCR Products
Cloning PCR Products
http://www.mcb.uct.ac.za//pcrcond.htm#Labelling
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What's PCR?
(Michael Blaber's Lab)
Illustrated introduction to various aspects of PCR technique. It's very valuable not only for those new to PCR, but also those familiar with PCR.
http://wine1.sb.fsu.edu/bch5425/lect22/lect22.htm
Added: Tue May 14 2002, Hits: 2040, Reviews: 0 Write review Cached