Subcategories

Related Categories:

• Real-Time PCR Primer

Protocols

• Real-Time RT-PCR Protocol (TIGR)
  This protocol describes how to perform real-time Reverse Transcription PCR in a two-step procedure (Reverse Transcription being a separate step from the PCR) using SYBR Green as the detected fluorophore and ROX as the passive reference.
  http://pga.tigr.org/sop/RT-PCR.pdf
  Added: Sun Jul 10 2005, Hits: 8392, Reviews: 0  Write review  
• RT-PCR: the Basic (Ambion)
  
  • Why Real-Time RT-PCR?
  • Real-Time PCR Chemistries
  • Quantitation of Results
  • Instrumentation for Real-Time PCR
  • Tools for Real-Time RT-PCR
  • End-Point RT-PCR: Relative vs. Competitive vs. Comparative
  • Relative RT-PCR
  • Competitive RT-PCR
  • Comparative RT-PCR
  • Tools for Any RT-PCR Technique
  
  http://www.ambion.com/techlib/basics/rtpcr/index.h...
  Added: Mon Mar 12 2007, Hits: 2283, Reviews: 0  Write review   Cached
• Troubleshooting Guide for qPCR and RTqPCR (Eurogentec)
  This troubleshooting guide takes you through the most common difficulties that you can meet when performing qPCR or RT qPCR assays using probes or SYBR® green I (Troubleshooting guide, probe and primers design guidelines).
  http://www.eurogentec.com/module/FileLib/GRT-TSGCU...
  Added: Mon Jul 12 2004, Hits: 8050, Reviews: 0  Write review  
• Two-Step Quantitative RT-PCR Protocol (GenScript.com)
  http://www.genscript.com/tech_guide/TM0170.pdf
  Added: Sun Jul 10 2005, Hits: 2722, Reviews: 0  Write review  
• Two-Step Real-Time RT-PCR (TIGR)
  This protocol describes how to perform real-time Reverse Transcription PCR in a twostep procedure (Reverse Transcription being a separate step from the PCR) using SYBR Green as the detected fluorophore and ROX as the passive reference.
  http://pga.tigr.org/sop/RT-PCR.pdf
  Added: Mon Dec 19 2005, Hits: 2096, Reviews: 0  Write review  

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