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Protocols
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RT-PCR procedures
(Roche Diagnostics Corporation)
RT-PCR can be performed as either a two-step (one tube or two tube) or an one-step (coupled RT-PCR) procedure. This protocol introduces both methods and their advantages.
http://www.roche-applied-science.com/pcr/applicati...
Added: Sat Sep 21 2002, Hits: 6929, Reviews: 0 Write review Cached -
RT-PCR Protocol
(Julie B. Wolf, University of Maryland, Baltimore County)
First strand synthesis and subsequent PCR amplification. Based on LTI protocol
http://userpages.umbc.edu/~jwolf/m12.htm
Added: Tue May 14 2002, Hits: 7053, Reviews: 0 Write review Cached -
RT-PCR Protocol
(NEB)
This kit instruction provides general and useful information on RT reactio and PCR such how to select priming methods.
http://www.neb.com/nebecomm/ManualFiles/manualE640...
Added: Mon Mar 12 2007, Hits: 493, Reviews: 0 Write review -
RT-PCR: the Basic
(Ambion)
- Why Real-Time RT-PCR?
- Real-Time PCR Chemistries
- Quantitation of Results
- Instrumentation for Real-Time PCR
- Tools for Real-Time RT-PCR
- End-Point RT-PCR: Relative vs. Competitive vs. Comparative
- Relative RT-PCR
- Competitive RT-PCR
- Comparative RT-PCR
- Tools for Any RT-PCR Technique
http://www.ambion.com/techlib/basics/rtpcr/index.h...
Added: Mon Mar 12 2007, Hits: 2268, Reviews: 0 Write review Cached