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Protocols
- Isabel’s Magical Cloning Protocol
(Lahn's Lab, University of Chicago)
Describes the whole molecular cloning procedures from PCR amplification, vector and insert digestion, vector dephosphorylation, ligation, transformation, plasmid preparation and screening to plasmid sequencing.
http://hominid.uchicago.edu/ProtocolPDFs/Cloningpr...
Added: Fri Mar 06 2009, Hits: 4571, Reviews: 0 Write review - Lance's Subcloning procedures (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Very informative and useful for all cloning procedures.
http://www.its.caltech.edu/%7Ebjorker/Martin_subcl...
Added: Wed Aug 14 2002, Hits: 2890, Reviews: 0 Write review - PROTOCOLS FOR RECOMBINANT DNA ISOLATION, CLONING, and SEQUENCING (Bruce A. Roe Lab, University of Oklahoma)
This manual is a compilation of many of the everyday methods used in the average molecular biology laboratory, with emphasis on the techniques for large scale DNA sequencing protocols and DNA sequencing automation techniques. The manual has been written in a protocol format, with little theoretical discussion.
http://www.genome.ou.edu/protocol_book/protocol_in...
Added: Tue Feb 03 2009, Hits: 3050, Reviews: 0 Write review Cached - Subtractive Cloning (Hazel Sive, MIT)
Subtractive cloning is a powerful technique that allows isolation of the differences in the nucleic acid composition of two cell samples (Figure 1). Differences can be at the level of RNA species represented within each sample or within the complement of genomic DNAs. Such differences include genes whose differential expression distinguishes one cell type from another, one growth phase from another, or a ormal state from a diseased state. A related procedure, termed positive selection, has been used to isolate differences in cDNA and in genomic DNAs among various genotypes.
http://web.wi.mit.edu/sive/pub/Lab%20Resources%20D...
Added: Sat May 24 2003, Hits: 1209, Reviews: 0 Write review -
An Introduction to Cloning
(Invitrogen.com)
Whether you’re looking for your gene of interest, already possess your starting nucleic acid, or are a few steps into your cloning experiment, this guide will help you successfully complete your cloning experiment. It contains a wealth of information about the products we offer for your cloning protocols.
http://www.invitrogen.com/site/us/en/home/Referenc...
Added: Wed May 18 2011, Hits: 1276, Reviews: 0 Write review -
Clone Genes From Phage Libraries
(Johnson Lab, FDept. Microbiology & Immunology, UCSF)
The overall sequence of events is: • Titer and plate out phage • Lift plaques onto filters and prepare them for screening • Make a probe • Hybridize the probe to the filters • Wash the filters and expose to film • Purify putative plaques • Excise plasmid from the desired phage
http://itsa.ucsf.edu/~micro/Faculty/Johnson/protoc...
Added: Fri Oct 17 2003, Hits: 115, Reviews: 0 Write review Cached -
Cloning of small RNAs with 5' phosphate and 3' OH ends
(Epigenome Network of Excellence)
Describes a procedure for the purification and cloning of miRNAs and other small RNAs in the 20-30 nucleotide size range from plant tissue.
http://www.epigenome-noe.net/researchtools/protoco...
Added: Wed May 27 2009, Hits: 363, Reviews: 0 Write review Cached -
DNA Cloning
(Clonit,Worthington)
Preparation of DNA Fragments for Insertion into Vector
Preparation of Cleaved Plasmid DNA
Ligation
Preparation of Competent Cells
Transformation
http://www.worthington-biochem.com/molecBio/clone_...
Added: Tue May 14 2002, Hits: 3483, Reviews: 0 Write review Cached -
DNA Cloning
(Fermentas)
Provides the following details: 1. Cleavage of the DNA vector.
2. Dephosphorylation of DNA vector cleaved at unique site.
3. DNA fragment preparation.
4. Filling-in of 5'-overhangs of the DNA fragment and/or vector termini.
5. Ligation.
6. Transformation.
7. Clone selection.
http://www.fermentas.com/Profiles/Kits/PDNAClonKit...
Added: Tue May 14 2002, Hits: 2000, Reviews: 0 Write review Cached -
PJB’s hints for sub-cloning
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Very useful tips for cloning
http://www.its.caltech.edu/%7Ebjorker/PJB%27s_clon...
Added: Wed Aug 14 2002, Hits: 1323, Reviews: 0 Write review -
Quick Shotgun Cloning of Phage Inserts
(Schneitz Lab)
Cloning DNA into phage vector
http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
Added: Tue May 14 2002, Hits: 800, Reviews: 0 Write review Cached -
Random Subclone Generation
(Roe Lab)
This is a complete protocol for the following procedures
DNA Sonication
DNA Nebulization
Random fragment end-repair, size selection, and phosphorylation
DNA ligation
Competent cell preparation
Bacterial cell transformation
Microcentrifuge tube transformation
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 1259, Reviews: 0 Write review Cached -
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
(Pamela Stanley Lab Wiki, Yale University)
The page explain how molecular cloning works from cloning gene into a plasmid, transformation to transfection.
http://stanxterm.aecom.yu.edu/wiki/index.php?page=...
Added: Thu Mar 26 2009, Hits: 1580, Reviews: 0 Write review Cached
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