Protocols
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Klenow Reaction
(Buck Institute)
Klenow fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'-> 5' exonuclease activity, but has lost 5'->3' exonuclease activity. This protocol describes how to set up and conduct a klenow reaction
http://www.protocol-online.org/cgi-bin/prot/view_c...
Added: Wed May 11 2005, Hits: 1902, Reviews: 0
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Large (Klenow) Fragment of E. coli DNA Polymerase I
(Dr. Bowen et al, Colorado State University)
The large or Klenow fragment of DNA polymerase I has DNA polymerase and 3' -> 5' exonuclease activities, and is widely used in molecular biology.
http://arbl.cvmbs.colostate.edu/hbooks/genetics/bi...
Added: Wed May 11 2005, Hits: 1384, Reviews: 0
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