Top : Molecular Biology : Enzymatic Manipulation of Nucleic Acid : Modification


Protocols
  • Phosphatase treatment of DNA fragments PDF file (Practical Approach Online, Oxford University Press)
    Includes treatment with bacterial alkaline phosphatase and calf intestinal phosphatase
    http://www.oup.co.uk/pdf/pas/9v1-7-8.pdf
    Added: Sat Aug 17 2002, Hits: 1124, Reviews: 0  Write review  
  • Alkaline phosphatase treatment of DNA (CIP) (Schneitz Lab)
    http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
    Added: Tue May 14 2002, Hits: 903, Reviews: 0  Write review   Cached
  • CIP Dephosphorylation of Linearized DNA (Vesicle Trafficking)
    http://cmgm.stanford.edu/~vesicle/protocols/cip.ht...
    Added: Tue May 14 2002, Hits: 755, Reviews: 0  Write review   Cached
  • Cleavage Efficiency Close to the Termini of PCR Fragments (Fermentas)
    When designing PCR experiments in which the synthesized DNA fragment is to be subsequently digested with a RE, it is very important to determine how many extra nucleotides should be added to the 5’-end of the PCR primer next to the introduced recognition site to ensure efficient cleavage with the appropriate restriction endonuclease. Some restriction endonucleases cleave DNA poorly when their recognition sites are located at the ends of DNA fragments. Information on restriction endonuclease performance close to the end of DNA fragments may be helpful when planning the introduction of cleavage sites at DNA termini in PCR experiments.
    http://www.fermentas.com/techinfo/RE/restrdigpcrii...
    Added: Tue May 14 2002, Hits: 382, Reviews: 0  Write review   Cached
  • De-phosphorylation of DNA (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
    Calf intestinal phosphatase (CIP) treatment of DNA and subsquently purification.
    http://www.nottingham.ac.uk/~mbzspd/methods/De-pho...
    Added: Sat Apr 10 2004, Hits: 81, Reviews: 0  Write review   Cached
  • Digestion of PCR Products (Fermentas)
    Some basic facts you need to know to cut your PCR products.
    http://www.fermentas.com/techinfo/RE/restrdigpcr.h...
    Added: Tue May 14 2002, Hits: 258, Reviews: 0  Write review   Cached
  • Phosphatasing with Shrimp Alkaline Phosphatase (S.A.P.) (Hedrick Lab, UCSD Cancer Center)
    Use to prevent linearized vector plasmid from recircularizing
    http://hedricklab.ucsd.edu/Protocol/SAP.html
    Added: Tue Dec 10 2002, Hits: 605, Reviews: 0  Write review   Cached