Related Categories:
Protocols
- DNA Sequential Digestion
(Practical Approach Online, Oxford University Press)
Sequential digestion of DNA using two restriction endonucleases
http://www.oup.co.uk/pdf/pas/9v1-7-2.pdf
Added: Sat Aug 17 2002, Hits: 6323, Reviews: 0 Write review - Restriction Digests of Genomic DNA (CIMMYT Applied Molecular Genetics Laboratory)
Very nice protocol for large scale digestion experiments, including some calculations, DNA precipitation after digestion.
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Fri Aug 02 2002, Hits: 5639, Reviews: 1 Read reviews Write review - Restriction enzyme digestion of DNA (basic method) (Matt Lewis, Department of Pathology, University of Liverpool)
Covers many aspects of restriction digestion experiments besides a typical protocol, such as how much DNA is needed, double digestion and star activity.
http://www.methodbook.net/dna/restrdig.html
Added: Fri Sep 06 2002, Hits: 12642, Reviews: 0 Write review Cached -
Alphabetic List of Commercial Restriction Endonucleases and Their Isoschizomers
(Fermentas)
http://www.fermentas.com/techinfo/RE/restrcommercr...
Added: Tue May 14 2002, Hits: 2453, Reviews: 0 Write review Cached -
Basics of Restriction Enzymes
(Advanced Molecular Biology Techniques Laboratory, Hofstra Univeristy, Hempstead)
How to handle restriction enzymes
http://www.people.hofstra.edu/faculty/Beverly_Clen...
Added: Tue Apr 26 2005, Hits: 2547, Reviews: 0 Write review Cached -
Cleavage Efficiency Close to the Termini of PCR Fragments
(Fermentas)
When designing PCR experiments in which the synthesized DNA fragment is to be subsequently digested with a RE, it is very important to determine how many extra nucleotides should be added to the 5’-end of the PCR primer next to the introduced recognition site to ensure efficient cleavage with the appropriate restriction endonuclease. Some restriction endonucleases cleave DNA poorly when their recognition sites are located at the ends of DNA fragments. Information on restriction endonuclease performance close to the end of DNA fragments may be helpful when planning the introduction of cleavage sites at DNA termini in PCR experiments.
http://www.fermentas.com/techinfo/RE/restrdigpcrii...
Added: Tue May 14 2002, Hits: 2091, Reviews: 0 Write review Cached -
Cleavage followed by fill-in of 5' overhangs to generate blunt ends.
(NEB)
http://www.neb.com/neb/tech/tech_resource/restrict...
Added: Tue May 14 2002, Hits: 768, Reviews: 0 Write review Cached -
Cleavage with two restriction endonucleases that produce blunt ends.
(NEB)
http://www.neb.com/neb/tech/tech_resource/restrict...
Added: Tue May 14 2002, Hits: 485, Reviews: 0 Write review Cached -
Cleavage with two restriction endonucleases that produce compatible overhangs.
(NEB)
http://www.neb.com/neb/tech/tech_resource/restrict...
Added: Tue May 14 2002, Hits: 621, Reviews: 0 Write review Cached -
Commercial Restriction Endonucleases Sorted by Generated DNA Ends
(Fermentas)
Enzymes Generating 5'-protruding Ends
Enzymes Generating 3'-protruding Ends
Enzymes Generating Blunt Ends
Enzymes Cleaving DNA on Both Sides of Their Recognition Sequence
http://www.fermentas.com/techinfo/RE/restrcommre_d...
Added: Tue May 14 2002, Hits: 2176, Reviews: 0 Write review Cached
Trouble Shootings and FAQs
- how can one decide the amount of DNA to be digested?
- Cut two closely located restriction sites
- Troubleshooting a double digestion of a standard yeast plasmid
- DNA refractory to digestion, why?
- Double digestion of a vector - how to verify the success since only 14 bp fragment is removed?
- Couldn't digest my vector with an insert - Could be the ClaI used is dam methylation sensitive?
- Difficulties with ligating digested pcr products to vectors
- Why my construct cannot be double cut?
- plasmid can't be double digested
- When to add salt after restriction digestion for phenol/chroloform purification
- Troubleshooting a double digestion problem - methylation, enzyme unit issues
- BamH1 and Nde1 double digestion problem
- BamH1 and Xho1 double digestion problem
- Problem in double digestion
- How to digest a vector with two restriction sites too close sited?
- Purify PCR products or not before restriction digest
- How do you inactivate the enzymes after digestion?