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Alkaline Agarose Gel Electrophoresis
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Alkaline agarose gels can be used to determine the size and quality of first and second strand cDNA synthesis by reverse transciptase.
http://www.nottingham.ac.uk/~mbzspd/methods/Alkali...
Added: Sat Apr 10 2004, Hits: 1271, Reviews: 0 Write review Cached -
DNA Mobility in Gels
(Roe, OU)
http://www.genome.ou.edu/protocol_book/protocol_ad...
Added: Tue May 14 2002, Hits: 2585, Reviews: 0 Write review Cached -
Electrophoresis of PCR products
(Vodkin Laboratory, University of Illinois)
Describes gel preparation, running and staining
http://soybeangenomics.cropsci.uiuc.edu/protocols/...
Added: Mon Aug 26 2002, Hits: 3051, Reviews: 0 Write review Cached -
Low cost DNA gel photography
(Nick Oswald, Bitesizebio.com)
How to assemble a DNA gel documentation apparatus using a standard digital camera.
http://bitesizebio.com/2007/08/29/low-cost-dna-gel...
Added: Wed Aug 29 2007, Hits: 173, Reviews: 0 Write review Cached -
Standard Neutral Agarose Electrophoresis
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Standard agarose gels can be prepared using either TBE or TAE running buffers.
http://www.nottingham.ac.uk/~mbzspd/methods/Standa...
Added: Sat Apr 10 2004, Hits: 1909, Reviews: 0 Write review Cached -
Top 10 Fun Facts for DNA Electrophoresis
(Produced from LTI)
Useful tips for preparing agarose gel and electrophoresis
http://www.bio.davidson.edu/courses/Molbio/tips/fu...
Added: Tue May 14 2002, Hits: 2925, Reviews: 0 Write review Cached -
Trouble Shooting DNA Agarose Gels
(Invitrogen)
No band, Smear, anomalies band migration? Find trouble shooting here
http://www.bio.davidson.edu/courses/Molbio/tips/tr...
Added: Tue May 14 2002, Hits: 2864, Reviews: 0 Write review Cached -
What to do if you mess up and run your agarose gel backwards
(IzziD wetlab)
If you wire your gel rig backwards causing your sample to migrate in the wrong direction, you can still recover your DNA and run your sample normally as long as the sample hasn't yet run off the edge of the gel. Simply reverse the wires to put them in the correct orientation (turn off the power supply first off course!), and run your gel normally. The samples will migrate past the loading wells and back into the correct portion of your gel. The separation should be fine, but very short bands may be slightly fainter due to diffusion.
http://wetlab.izzid.com/2007/Oct/What_to_do_Agaros...
Added: Sat Oct 27 2007, Hits: 163, Reviews: 0 Write review