Top : Molecular Biology : DNA : Mutagenesis

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Protocols
  • Generation of Uni-directional Nested Deletions (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
    Making uni-directional nested deletion in plasmid DNA.
    http://www.nottingham.ac.uk/~mbzspd/methods/Genera...
    Added: Sat Apr 10 2004, Hits: 130, Reviews: 0  Write review   Cached
  • Creating a Seletion by PCR Splicing (Methods.info)
    To delete a desired fragment from existing DNA fragment all you need is a pair of primers combined of flanking sequences and a high fidelity polymerase.
    http://www.methods.info/Tips/mutagenesis/PCR_splic...
    Added: Sat Dec 20 2003, Hits: 1316, Reviews: 0  Write review   Cached
  • Deletion Mutagenesis (Promega)
    For construction of plasmid or M13 subclones containing progressive unidirectional deletions of inserted DNA, Including methods for DNA preparation, vector digestion, exonuclease deletion, ligation, transformation...
    http://www.promega.com/tbs/tm006/tm006.html#i
    Added: Tue May 14 2002, Hits: 2442, Reviews: 0  Write review   Cached
  • DpnI Mediated Site-Directed Mutagenesis (Nonet Lab - Washington University School of Medicine)
    A highly effective simple method for making site directed lesions in plasmids without subcloning.
    http://thalamus.wustl.edu/nonetlab/ResourcesF/dpnm...
    Added: Sat Nov 01 2003, Hits: 2709, Reviews: 0  Write review   Cached
  • Dut/ung in vitro Site-directed Mutagenesis PDF file (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
    Detailed procedures
    http://www.its.caltech.edu/%7Ebjorker/Site-directe...
    Added: Wed Aug 14 2002, Hits: 1428, Reviews: 0  Write review  
  • Exo/S1 Deletion Series (Vesicle Trafficking)
    http://cmgm.stanford.edu/~vesicle/protocols/exo_s1...
    Added: Tue May 14 2002, Hits: 757, Reviews: 0  Write review   Cached
  • ExoIII/S1 Deletion (Fermentas)
    For construction of plasmid or M13 subclones with nested unidirectional deletions. The method is applicable for DNA sequencing, mapping of boundaries of regions involved in genetic control, DNA/protein interaction sites, or protein domains.
    http://www.fermentas.com/Profiles/Kits/PExoIIIKit....
    Added: Tue May 14 2002, Hits: 96, Reviews: 0  Write review   Cached
  • In vitro Mutagenesis Using Altered Sites (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
    http://www.pmci.unimelb.edu.au/core_facilities/man...
    Added: Tue May 14 2002, Hits: 162, Reviews: 0  Write review   Cached
  • In Vitro Mutagenesis Using Altered Sites (Bowtell Lab)
    http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
    Added: Tue May 14 2002, Hits: 112, Reviews: 0  Write review   Cached
  • In vitro Mutagenesis with dut ung Single Stranded DNA (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
    http://labs.fhcrc.org/hahn/Methods/mol_bio_meth/in...
    Added: Tue May 14 2002, Hits: 783, Reviews: 0  Write review   Cached
  • Mutagenesis (Chen)
    Two methods are introduced. The first one is more labourious (~a week) but is cheap. The second method is easy and quick (1-2 days) but more expensive.
    http://www.biochem.ucl.ac.uk/~chen/protocols/mutag...
    Added: Tue May 14 2002, Hits: 2944, Reviews: 0  Write review  
  • Nested Deletions (Bowtell Lab)
    Nested Deletions using exonuclease-III and mung bean nuclease
    http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
    Added: Tue May 14 2002, Hits: 140, Reviews: 0  Write review   Cached
  • Nested Deletions Using Exonuclease-III and Mung Bean Nuclease (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
    http://www.pmci.unimelb.edu.au/core_facilities/man...
    Added: Tue May 14 2002, Hits: 99, Reviews: 0  Write review   Cached
  • Nested Deletions Using Exonuclease-III and Mung Bean Nuclease (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
    http://www.pmci.unimelb.edu.au/core_facilities/man...
    Added: Tue May 14 2002, Hits: 85, Reviews: 0  Write review   Cached
  • Nested-Deletion Protocol (Laurie Lab, University of Virginia Health Science Center)
    http://people.virginia.edu/~gwl6s/home.html/Method...
    Added: Tue May 14 2002, Hits: 1023, Reviews: 0  Write review   Cached
  • Oligonucleotide-directed mutagenesis (Kunkel method) (Jeffrey D. Newman, Lycoming College)
    http://srv2.lycoming.edu/~newman/courses/molbiol/l...
    Added: Tue May 14 2002, Hits: 1138, Reviews: 0  Write review   Cached
  • Site Directed Mutagenesis Using the Ung-Dut System (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
    http://www.pmci.unimelb.edu.au/core_facilities/man...
    Added: Tue May 14 2002, Hits: 134, Reviews: 0  Write review   Cached
  • Site-directed Mutagenesis (Promega)
    For generation and selection of oligonucleotide-directed mutants
    http://www.promega.com/tbs/tm001/TM001.html#v
    Added: Tue May 14 2002, Hits: 1997, Reviews: 0  Write review   Cached
  • Targeted Amplification of Mutant Strand (TAMS) for Site Directed Mutagenesis (Lei Young)
    Targeted Amplification of Mutant Strand (TAMS) technology allows multiple-site mutagenesis in a simple, half-day protocol. It’s the only method that can mutate first strand cDNA directly, and perform multiple-site mutagenesis at high efficiency in a single round. TAMS technology takes advantage of the fact that primers with 3’ mismatches usually cannot amplify the target sequence. Therefore, if two anchor mutations are incorporated into the mutant DNA strand outside the desired mutations, they can be preferentially amplified by primers with 3’ sequence matching the mutant strand but not the wild-type strand. Since the desired mutations are in the mutant strand, the final PCR product will also contain the desired mutations.
    http://ozex.netfirms.com/TS.html
    Added: Tue Nov 26 2002, Hits: 144, Reviews: 0  Write review   Cached
  • Targeted Amplification of Mutant Strand (TAMS) technology for site directed mutagenesis (iProtocol)
    Targeted Amplification of Mutant Strand (TAMS) technology allows multiple-site mutagenesis in a simple, half-day protocol. It’s the only method that can mutate first strand cDNA directly, and perform multiple-site mutagenesis at high efficiency in a single round.
    http://iprotocol.mit.edu/protocol/330.htm
    Added: Fri Oct 17 2003, Hits: 107, Reviews: 0  Write review   Cached
  • Tn5 Mutagenesis (E. coli Genome Project at UW-Madison )
    Mutate E.coli K12 genes with Tn5 Transposon
    http://www.genome.wisc.edu/resources/protocols/tnm...
    Added: Tue Aug 06 2002, Hits: 1921, Reviews: 0  Write review   Cached